{"product_id":"abcam-ab263396","title":"Abcam, ab263396, Human TOR3A knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTOR3A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1 and 1 bp insertion in exon1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1 and 1 bp insertion in exon1.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TOR3A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTOR3A also known as torsin-3A is a member of the torsin family of proteins. It has a molecular weight of approximately 39 kDa. This protein mainly localizes to the endoplasmic reticulum and nucleus where it plays a role in maintaining cellular integrity. TOR3A is involved in the regulation of protein folding ensuring that proteins attain and maintain their correct conformation. Its expression is found in various tissues but is particularly notable in neural tissues where proper protein folding is imperative for cell function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe protein participates in processes related to maintaining cellular homeostasis. Although not a direct component of large protein complexes TOR3A interacts with molecular chaperones to assist in the correct folding and assembly of polypeptides. This function is foundational for maintaining cell survival and minimizing stress responses. The protein may interact with elements of the cell's quality control systems to facilitate the degradation of misfolded proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nTOR3A takes part in cellular stress response and protein quality control pathways. It functions alongside other proteins like heat shock proteins (HSPs) to modulate the folding and repair of cellular proteins ensuring that they are correctly synthesized and functional. These pathways are key to cellular homeostasis helping cells to cope with stress and alterations in their environment.\u003cbr\u003e\nTOR3A has links to certain neurological disorders where protein misfolding occurs. Abnormal functioning of TOR3A can result in the accumulation of misfolded proteins contributing to conditions such as neurodegenerative diseases like Alzheimer's. It may also relate to dystonia a movement disorder characterized by involuntary muscle contractions where its interaction with TOR1A or torsin-1A becomes significant. These associations highlight the importance of protein quality control mechanisms in preventing disease conditions related to neural tissues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845168943273,"sku":"ab263396","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263396","provider":"Iright","version":"1.0","type":"link"}