{"product_id":"abcam-ab263400","title":"Abcam, ab263400, Human TSSK3 (TSSK-3) knockout HeLa cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nTSSK3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon2.,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TSSK3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTSSK-3 also known as Testis-Specific Serine Kinase 3 is a protein kinase that performs phosphorylation. It has a mass of approximately 37 kDa. This protein is primarily expressed in the testis where it plays a role in testicular function. TSSK-3 belongs to the family of serine\/threonine kinases and is involved in signaling processes specific to sperm development and function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSerine\/threonine kinase activities of TSSK-3 influence spermatogenesis. TSSK-3 engages in the regulation of important processes like cell cycle control and differentiation required for sperm maturation. Although TSSK-3 does not form part of an extensive complex its function notably impacts cellular activities during sperm development.\u003cbr\u003e\nPathways\u003cbr\u003e\nTSSK-3 function integrates within the MAPK signaling pathway and the cell cycle regulation pathway. In the MAPK signaling pathway it interacts with other kinases and signaling molecules that modulate testicular functions and sperm maturation. Within cell cycle regulation TSSK-3 associates with proteins that govern cell division and polarization such as CDKs (Cyclin Dependent Kinases).\u003cbr\u003e\nTSSK-3 links closely to male infertility and defects in spermatogenesis. Mutations or dysregulation in TSSK-3 activity can impact fertility by impairing sperm development. Additionally TSSK-3 may co-function with proteins like Aurora Kinases which also influence cell cycle processes contributing to testicular anomalies affecting fertility potential.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845214294185,"sku":"ab263400","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263400","provider":"Iright","version":"1.0","type":"link"}