{"product_id":"abcam-ab263429","title":"Abcam, ab263429, Human ZC3H15 knockout HEK-293T cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nZC3H15 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ZC3H15, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nZinc finger CCCH-type containing 15 (ZC3H15) is a small protein with a mass of approximately 70 kDa. The protein has an additional name NDP52 that arises from research in specific contexts of cell biology. ZC3H15 contains a zinc finger domain that binds RNA indicating its role in post-transcriptional regulation. The protein shows expression in multiple cell types but higher expression levels occur in immune cells reflecting its specialized functions in immunity and cellular signaling.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nZC3H15 participates in the regulation of mRNA stability and degradation impacting gene expression dynamically. The protein acts as part of a ribonucleoprotein complex working together with other proteins to influence RNA processing events. ZC3H15 modulates innate immune responses by controlling the degradation and translation of mRNAs encoding inflammatory mediators. This regulation helps maintain cellular homeostasis during immune challenges.\u003cbr\u003e\nPathways\u003cbr\u003e\nZC3H15 plays significant roles in the NF-kB signaling and apoptosis pathways. It influences the NF-kB pathway by interacting with proteins that control inflammatory gene expression such as TRAF2 and TAK1. ZC3H15 affects the apoptotic pathway through its impact on mRNA stability altering the expression of key apoptotic regulators. These interactions showcase its functional importance in balancing cell survival and death decisions under stress and pathological conditions.\u003cbr\u003e\nZC3H15 has associations with autoimmune conditions and infectious diseases. Dysregulation of ZC3H15 can lead to excessive inflammation contributing to diseases like rheumatoid arthritis through mishandled NF-kB signaling. In infectious diseases the modulation of ZC3H15 expression links to viral pathology as certain viruses exploit this protein to evade immune responses. Exploring these connections could inform potential therapies targeting ZC3H15 or its associated pathways involving proteins like MAVS that play central roles in antiviral defense.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855777419433,"sku":"ab263429","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263429","provider":"Iright","version":"1.0","type":"link"}