{"product_id":"abcam-ab263881","title":"Abcam, ab263881, Human AST ELISA Kit (Aspartate Aminotransferase)","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests\u003cbr\u003e\nHuman AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min Simplestep used to quantify Human AST (Aspartate Aminotransferase) with a sensitivity of 43 pg\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 43 pg\/mL,\u003cbr\u003e\nRange:312.5 - 20000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman AST ELISA Kit (Aspartate Aminotransferase) ab263881 is a rapid single-wash 90-min ELISA kit to measure Human AST in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 43 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman AST SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit is designed for the quantification of human AST. The standard protein in this kit is full length human AST. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), cell culture supernatant, cell extract and tissue extract sample types. Saliva, urine, milk, and CSF samples have not been tested with this kit.\u003cbr\u003e\nHuman AST ELISA Kit (Aspartate Aminotransferase) ab263881 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human AST ELISA Kit (Aspartate Aminotransferase) ab263881\u003cbr\u003e\nHuman AST ELISA Kit (Aspartate Aminotransferase) ab263881 has been used to study oxidative and inflammatory responses to smoking\u003cbr\u003e\n, blood biomarker expression in acute pancreatitis\u003cbr\u003e\n, and human Liver-Chip as a model for predicting drug-induced liver injury\u003cbr\u003e\nReferences:\u003cbr\u003e\nHande Sipahi et al. 2023, PMID: 36650104,\u003cbr\u003e\nHuaming Ding et al. 2022, PMID: 36816737,\u003cbr\u003e\nJaney Ronxhi, et al. 2019, PMID: 35790095\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human AST ELISA Kit (Aspartate Aminotransferase) only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human\/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free\u003cbr\u003e\nab267701\u003cbr\u003e\n- Anti-Aspartate Aminotransferase antibody [EPR20781-274] - BSA and Azide free (Capture)\u003cbr\u003e\nab259691\u003cbr\u003e\n- Anti-Aspartate Aminotransferase antibody [EPR20781-282] - BSA and Azide free (Detector)\u003cbr\u003e\nab259692\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman AST ELISA Kit (Aspartate Aminotransferase) ab263881 is commonly used by researchers to measure liver function.\u003cbr\u003e\nOther ELISA kits to measure liver function include:\u003cbr\u003e\n- Human ALT ELISA kit\u003cbr\u003e\nab234578\u003cbr\u003e\n- Human Albumin ELISA kit\u003cbr\u003e\nab179887\u003cbr\u003e\n- Albumin (BCG) Assay Kit (Colorimetric)\u003cbr\u003e\nab235628\u003cbr\u003e\nThese ELISA kits are also available for Mouse\/Rat:\u003cbr\u003e\n- Mouse AST ELISA kit\u003cbr\u003e\nab263882\u003cbr\u003e\n- Mouse ALT ELISA kit\u003cbr\u003e\nab282882\u003cbr\u003e\n- Mouse Albumin ELISA kit\u003cbr\u003e\nab207620\u003cbr\u003e\n- Rat AST ELISA kit\u003cbr\u003e\nab263883\u003cbr\u003e\n- Rat ALT ELISA kit abab234579\u003cbr\u003e\n- Rat Albumin ELISA kit\u003cbr\u003e\nab235642\u003cbr\u003e\nCytoplasmic aspartate aminotransaminase (AST), is a key enzyme in the regulation of glutamate levels. It catalyzes the reversible transfer of an a-amino group between aspartate and glutamate. Given its central role in amino acid metabolism, it plays a critical role in many biological functions. In a clinical context, AST levels in the serum are used as a marker for liver health. This kit targets the full-length protein.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843552956585,"sku":"ab263881","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab263881","provider":"Iright","version":"1.0","type":"link"}