{"product_id":"abcam-ab264625","title":"Abcam, ab264625, Human TrkB ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman TrkB ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TrkB with a sensitivity of 23.76 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Extracts,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 23.76 pg\/mL,\u003cbr\u003e\nRange:54.69 - 3500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman TrkB ELISA Kit ab264625 is a rapid single-wash 90-min Sandwich ELISA to measure Human TrkB in tissue extracts. This SimpleStep sensitivity is 23.76 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman TrkB SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman TrkB ELISA Kit ab264625 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-TrkB antibody [EPR18925-15] - BSA and Azide free (Capture)\u003cbr\u003e\nab242343\u003cbr\u003e\n- Anti-TrkB antibody [EPR18925-48] - BSA and Azide free (Detector)\u003cbr\u003e\nab242343\u003cbr\u003e\nTrkB, also known as NTRK2, is a tyrosine kinase receptor involved in the development of the peripheral and central nervous system. TrkB plays a role in synapse plasticity and formation, and neuron survival, proliferation and differentiation. Ligand (including BDNF and NTF4) interactions induces homodimerization, autophosphorylation and activation of TrkB. This kit targets an N-terminal fragment of the mature chain and shares approximately 90% sequence identity with mouse and rat.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nTrkB is a pivotal player in both the MAPK\/ERK and PI3K\/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLC? interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.\u003cbr\u003e\nTrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843568717993,"sku":"ab264625","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab264625","provider":"Iright","version":"1.0","type":"link"}