{"product_id":"abcam-ab265100","title":"Abcam, ab265100, Human SNRPB2 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSNRPB2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SNRPB2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSNRPB2 also known as small nuclear ribonucleoprotein polypeptide B' is a protein that plays a role in the splicing of pre-mRNA. It often weighs around 25 kDa and is expressed in the nucleus of cells. The protein is a component of the spliceosome which is an essential part of the RNA processing machinery in eukaryotic cells. In particular SNRPB2 is part of the U2 small nuclear ribonucleoprotein (snRNP) complex acting in spliceosome assembly and RNA splicing events.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProteins like SNRPB2 contribute to the regulation of gene expression by ensuring the accurate removal of introns from precursor mRNA. SNRPB2 exists as part of the U2 snRNP an important component of the spliceosome. This complex participates in recognizing the splicing sites and catalyzing the chemical reactions necessary for intron excision. Therefore SNRPB2 along with other proteins and RNA molecules within the spliceosome guarantees precise RNA maturation which is necessary for correct protein synthesis in eukaryotic cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nRNA splicing machinery like the spliceosome featuring SNRPB2 engages in the RNA processing pathway. This protein often works alongside U1 snRNP and is also interconnected with mRNA surveillance mechanisms ensuring that errors in RNA transcripts are recognized and degraded. Importantly SNRPB2 has relationships with other spliceosome components such as SF3A and SF3B that also ensure the fidelity of the splicing process and its integration with various cellular signaling pathways.\u003cbr\u003e\nMutations or malfunctions in SNRPB2 have links with conditions like retinitis pigmentosa and spinal muscular atrophy. These genetic disorders often stem from disruptions in the normal splicing processes that are essential for nerve and photoreceptor cell function. In these disorders connections have been made between SNRPB2 and other related proteins such as SMN1 in spinal muscular atrophy further illustrating the complexity of its involvement in maintaining healthy cellular function and gene expression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845212164265,"sku":"ab265100","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265100","provider":"Iright","version":"1.0","type":"link"}