{"product_id":"abcam-ab265242","title":"Abcam, ab265242, Human PSRC1 (DDA3) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPSRC1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: Insertion of the selection cassette in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PSRC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe PSRC1 protein also known as DDA3 plays an important role in cell cycle regulation. It is a microtubule-associated protein with a mass of approximately 33 kDa. PSRC1 is widely expressed in various tissues including the brain heart and liver. It interacts directly with microtubule plus-end tracking proteins and regulates microtubule dynamics which is essential for proper mitotic spindle function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPSRC1 plays an important role in ensuring correct chromosome segregation during cell division. It functions as part of the mitotic spindle assembly complex and is responsible for promoting stable attachment of microtubules to kinetochores which is critical for accurate chromosomal alignment. These functions involve interactions with proteins related to spindle formation and checkpoint signaling.\u003cbr\u003e\nPathways\u003cbr\u003e\nPSRC1 acts in the mitotic spindle checkpoint pathway and the microtubule dynamics pathway. Its interaction with proteins like CLASP1 and APC important for microtubule stability and dynamics highlights its contribution to maintaining proper mitotic progression. PSRC1 ensures successful completion of mitosis by coordinating these pathways important for cell division fidelity.\u003cbr\u003e\nPSRC1 connects to various types of cancer such as breast and colorectal cancer. It influences tumor progression through its effect on cell division errors and genomic instability. Additionally PSRC1 interacts with TP53 a protein responsible for cell cycle regulation and apoptosis suggesting its potential role in tumor suppression mechanisms compromised in cancerous conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845196206249,"sku":"ab265242","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265242","provider":"Iright","version":"1.0","type":"link"}