{"product_id":"abcam-ab265314","title":"Abcam, ab265314, Human SLC25A5 (Adenine Nucleotide Translocator 2\/ANT 2) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSLC25A5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC25A5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Adenine Nucleotide Translocator 2 (ANT 2) also referred to as ANT protein or 2 ANT is an important component of the mitochondrial inner membrane. It belongs to the family of adenine nucleotide translocator proteins with a molecular weight of about 33 kDa. ANT 2 facilitates the exchange of ATP and ADP across the mitochondrial membrane. This protein is mainly expressed in tissues with high energy demand such as the heart liver and skeletal muscle.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nANT 2 functions to regulate cellular energy metabolism by controlling the exchange of adenine nucleotides between the mitochondria and cytosol. It is a part of the permeability transition pore complex which plays a role in maintaining mitochondrial membrane potential and preventing apoptosis. ANT 2 interacts with nucleotides to modulate energy production making it essential for the maintenance of cellular bioenergetics.\u003cbr\u003e\nPathways\u003cbr\u003e\nANT 2 integrates into significant energy production pathways including oxidative phosphorylation. It forms a functional unit with proteins like the voltage-dependent anion channel (VDAC) within the mitochondrial interface. This relationship with VDAC is key to ATP production and involves cellular energy transactions fundamental to numerous metabolic and signaling pathways.\u003cbr\u003e\nANT 2 demonstrates relevance to both cancer and cardiomyopathy. In cancer the overexpression of ANT 2 may support the high energy demands of rapidly proliferating cells. Meanwhile in cardiomyopathy mutations or dysregulation in ANT 2 impact energy metabolism leading to heart dysfunction. The interaction of ANT 2 with pro-apoptotic proteins like Bax influences the pathogenesis of these diseases by mediating mitochondrial-induced apoptosis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845160784041,"sku":"ab265314","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265314","provider":"Iright","version":"1.0","type":"link"}