{"product_id":"abcam-ab265507","title":"Abcam, ab265507, Human RIN1 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nRIN1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RIN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRIN1 also known as Ras and Rab interactor 1 is a protein that plays a role in intracellular signaling. It has a molecular mass of approximately 80 kDa. RIN1 interacts with both Ras and Rab GTPases and acts as a guanine nucleotide exchange factor (GEF) for these proteins. In terms of expression researchers have detected RIN1 in various tissues including the brain and embryonic fibroblasts indicating its involvement in multiple cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRIN1 modulates signaling pathways by influencing the activation state of Ras-related GTPases. It forms part of a larger signaling complex that regulates endocytosis and trafficking of receptors. This activity is essential for receptor-mediated signaling and cellular communication. By affecting these processes RIN1 contributes significantly to the regulation of cytoskeletal dynamics which impacts cell movement and morphology.\u003cbr\u003e\nPathways\u003cbr\u003e\nRIN1 is directly involved in the Ras signaling pathway which is important for cell growth and differentiation. Through its interaction with Ras proteins RIN1 influences downstream effectors like Raf kinases and PI3K which further modulate signaling cascades. RIN1 also participates in the regulation of other pathways linked to receptor internalization partnering with proteins such as Rab5 to coordinate endocytic trafficking.\u003cbr\u003e\nDisruptions or alterations in RIN1 function have been associated with certain cancers notably lung and breast cancer. These associations highlight its role in abnormal cell proliferation and survival. Moreover RIN1's interactions with Ras proteins correlate with mutations that lead to oncogenic transformations. Understanding RIN1's normal and pathological roles helps reveal potential therapeutic targets for diseases related to Ras-driven signaling dysregulation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845164880041,"sku":"ab265507","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265507","provider":"Iright","version":"1.0","type":"link"}