{"product_id":"abcam-ab265559","title":"Abcam, ab265559, Human TBC1D7 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTBC1D7 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon 2 and 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 19 bp deletion in exon 2 and 2 bp deletion in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TBC1D7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTBC1D7 also known as TRE2-BUB2-CDC16 domain family member 7 is a protein with an approximate mass of 25 kDa. This protein acts as a GAP (GTPase-activating protein) for Rab small GTPases which are involved in intracellular trafficking. The expression of TBC1D7 occurs in various tissues such as the liver skeletal muscle and adipose tissue. With its primary role as a GAP TBC1D7 is significant in regulating Rab GTPases' cycling between active and inactive states affecting vesicular transport and cellular logistics.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTBC1D7 functions as a part of the TSC complex consisting of TSC1 (hamartin) TSC2 (tuberin) and TBC1D7. This complex plays a significant role in cellular growth regulation by inactivating the small GTPase Rheb. This action prevents the overactivation of mTORC1 (mechanistic target of rapamycin complex 1) an important controller of cell growth and proliferation. TBC1D7 adds stability to the TSC complex enhancing its ability to regulate mTORC1 activity effectively demonstrating its key role in cellular metabolic regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe involvement of the TBC1D7 protein in the mTOR signaling and insulin signaling pathways is well-documented. Within these pathways TBC1D7 operates along with proteins such as Rheb and mTOR itself. By affecting the TSC complex's inhibitory impact on mTORC1 TBC1D7 influences cell growth survival and metabolism. These pathways are essential for energy sensing and management impacting how cells respond to nutrient availability and growth signals.\u003cbr\u003e\nTBC1D7 has been linked to conditions like Smith-Kingsmore syndrome and tuberous sclerosis complex. In these disorders the TSC complex's ability to regulate mTOR signaling is disrupted. Mutations or dysregulation of TSC1 TSC2 or TBC1D7 itself can lead to unchecked mTOR activation resulting in abnormal cell growth and proliferation. The relationship between TBC1D7 and these conditions highlights its role beyond cellular mechanics influencing pathological states when its function is altered.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845152428201,"sku":"ab265559","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265559","provider":"Iright","version":"1.0","type":"link"}