{"product_id":"abcam-ab265611","title":"Abcam, ab265611, Human GNPAT (DAPAT) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nGNPAT KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 3 and 1 bp deletion in exon 3 and 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 3 and 1 bp deletion in exon 3 and 1 bp insertion in exon 3,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-GNPAT, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGNPAT also known as glyceronephosphate O-acyltransferase or dihydroxyacetone phosphate acyltransferase (DAPAT) is an enzyme that plays an important role in lipid metabolism. The enzyme has a mass of approximately 73 kDa. It is expressed mainly in peroxisomes organelles involved in various metabolic functions. The enzyme catalyzes the initial and rate-limiting step in the biosynthesis of ether lipids like plasmalogens by converting dihydroxyacetone phosphate (DHAP) to acyl-DHAP.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGNPAT is essential in lipid biosynthesis since ether lipids are integral components of cell membranes and signaling molecules. The enzyme does not work in isolation; it forms part of a multi-enzyme complex that includes other important proteins like alkylglycerone phosphate synthase (AGPS). This association allows GNPAT to efficiently catalyze the first steps in the formation of plasmalogens which serve key roles in protecting cells from oxidative stress and in cell signaling.\u003cbr\u003e\nPathways\u003cbr\u003e\nGNPAT functions efficiently within the ether lipid synthesis pathway which is critical for normal cellular membrane composition and function. The activity of GNPAT impacts the levels of plasmalogens influencing pathways involved in membrane dynamics and antioxidant defense. It works closely with AGPS enhancing the production of essential ether lipids and affects phospholipid metabolism interfacing indirectly with proteins involved in peroxisomal and mitochondrial functions.\u003cbr\u003e\nGNPAT dysfunction has associations with rhizomelic chondrodysplasia punctata (RCDP) a genetic disorder characterized by skeletal abnormalities and developmental delay. The enzyme's interaction with proteins like Phospholipase A2 is relevant in understanding its role in RCDP. Additionally alterations in plasmalogen levels due to impaired GNPAT function are observed in neurological conditions such as Alzheimer's disease. This connection highlights the importance of GNPAT in maintaining normal brain lipid metabolism and preventing cognitive decline.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845164585129,"sku":"ab265611","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265611","provider":"Iright","version":"1.0","type":"link"}