{"product_id":"abcam-ab265739","title":"Abcam, ab265739, Human C11orf58 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSMAP KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp deletion in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SMAP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSMAP (Small Arf-GTPase Activating Proteins) plays a role in the regulation of Arf GTPases and exhibits GTPase-activating protein (GAP) activity. SMAP also known as SMAP1 and SMAP2 is associated with endocytic and membrane traffic processes. The two main isoforms SMAP1 and SMAP2 have molecular masses of approximately 100 kDa and are expressed mainly in the cytoplasm of various cell types including those in the brain liver and kidney.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSMAP influences cellular processes by modulating membrane trafficking dynamics. SMAP operates as part of a complex involved in the regulation of vesicular transport. By assisting the hydrolysis of GTP bound to Arf proteins SMAP can control vesicle budding and transport at the Golgi apparatus. This function enables SMAP to participate actively in managing intracellular transport and membrane curvature events impacting cell signaling and other cellular activities.\u003cbr\u003e\nPathways\u003cbr\u003e\nSMAP functions in the clathrin-mediated endocytosis pathway and the Golgi transport pathway. Through its activity SMAP links to the Arf family proteins which are components in these pathways. In clathrin-mediated endocytosis SMAP facilitates cargo selection and vesicle formation interacting with molecules like AP-2. SMAP's GAP activity is important in Golgi-associated vesicular trafficking where it regulates the cycle of Arf GTPases contributing to vesicle docking and fusion events.\u003cbr\u003e\nAbnormalities in SMAP function can influence conditions such as cancer and neurodegenerative diseases. Alterations affecting SMAP expression or activity may lead to disrupted membrane trafficking and defective signal transduction pathways that are related to cancer metastasis. Additionally connections with Arf proteins link SMAP to neurological diseases due to impaired synaptic vesicle cycling and transport highlighting its significance in maintaining cellular homeostasis in neural tissues.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845203284137,"sku":"ab265739","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265739","provider":"Iright","version":"1.0","type":"link"}