{"product_id":"abcam-ab265796","title":"Abcam, ab265796, Human ASAP1 (DDEF1) knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nASAP1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ASAP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nASAP1 also known as DDEF1 stands for ADP-Ribosylation Factor GTPase-Activating Protein 1 and has a molecular weight of approximately 130 kDa. It plays a mechanical role in actin cytoskeleton remodeling by regulating ARF (ADP-ribosylation factor) GTPases. These are involved in intracellular vesicular transport. ASAP1 localizes to the cytoplasm and shows high expression in invasive cancer cell lines with noticeable expression noted in tissues such as the brain liver and lung.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nASAP1 interacts with components of the focal adhesion complex a group of proteins critical in signaling pathways that connect the extracellular matrix and the actin cytoskeleton. It modulates cell adhesion migration and invasion. ASAP1 binds to proteins like paxillin which further influences the dynamics of actin and membrane trafficking important for cellular rearrangements.\u003cbr\u003e\nPathways\u003cbr\u003e\nASAP1 plays roles in actin cytoskeleton reorganization pathways and integrin-mediated signaling pathways. These pathways are essential for cell motility and shape. In these contexts ASAP1 interacts with cortactin an actin-binding protein involved in the regulation of cytoskeletal dynamics necessary for the migration and invasion of cancer cells.\u003cbr\u003e\nASAP1 has associations with cancer progression particularly in breast and colorectal cancer. Research indicates that elevated ASAP1 expression correlates with aggressive tumor behavior and poor prognosis. Its interaction with paxillin during disease conditions highlights its involvement in tumor invasiveness and metastasis highlighting its potential as a therapeutic target in oncology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845155836073,"sku":"ab265796","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265796","provider":"Iright","version":"1.0","type":"link"}