{"product_id":"abcam-ab265848","title":"Abcam, ab265848, Human ZRANB1 knockout HeLa cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nZRANB1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HeLa,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Cervix,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1,\u003cbr\u003e\nDisease:Adenocarcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ZRANB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nZRANB1 also known as TRABID is a zinc finger RANBP2-type containing 1 protein with a mass of approximately 89 kDa. This protein functions majorly as a deubiquitinating enzyme specializing in cleaving Lys29- and Lys33-linked polyubiquitin chains. It is expressed across various tissues with notable expression in the human brain indicating its importance in neural functions. Mechanically ZRANB1 contains several zinc finger motifs which facilitate its interaction with ubiquitin substrates playing a critical role in protein ubiquitination homeostasis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nZRANB1 acts in the regulation of protein degradation and turnover impacting various cellular processes like signal transduction and cell cycle control. It functions as part of the ubiquitin-proteasome system which is a large multi-protein complex responsible for maintaining cellular protein homeostasis. Through its activity ZRANB1 influences not only regular cellular operations but also responds to stress signals by adjusting the degradation of proteins therefore impacting numerous physiological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nZRANB1 participates in the Wnt signaling pathway and the NF-kB pathway both of which are important for cell proliferation and immune response regulation. In the Wnt pathway it regulates the stability and signaling activity of key proteins like beta-catenin. Meanwhile ZRANB1's action in the NF-kB pathway involves modulating the degradation of ubiquitinated proteins with relations to proteins like NEMO. These pathways highlight the specific regulatory roles played by ZRANB1 within signaling networks.\u003cbr\u003e\nZRANB1 has been implicated in cancer and neurological disorders. In cancer aberrant regulation or mutations in ZRANB1 can lead to uncontrolled cell growth due to misregulation of the Wnt signaling pathway. In neurological disorders potential links to proteins involved in neural development highlight its role in maintaining neural homeostasis. By influencing pathways connected to these diseases ZRANB1 interacts with other proteins such as APC in cancer and possibly tau proteins in neurodegenerative disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845196304553,"sku":"ab265848","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab265848","provider":"Iright","version":"1.0","type":"link"}