{"product_id":"abcam-ab266122","title":"Abcam, ab266122, Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSLC25A10 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 7 bp deletion in exon 1 and Insertion of the selection cassette in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SLC25A10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe mitochondrial dicarboxylate carrier also known as the dicarboxylate transporter facilitates the exchange of dicarboxylates across the mitochondrial inner membrane. This carrier protein often termed the dicarboxylate carrier or oxodicarboxylate transporter possesses a molecular mass of approximately 31 kilodaltons and is expressed predominantly in metabolically active tissues such as liver kidney and heart. Its key function lies in transporting four-carbon dicarboxylates like succinate and malate important for energy and metabolic equilibrium.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe mitochondrial dicarboxylate carrier aids in mitochondrial processes by mediating the shuttle of dicarboxylates important for the tricarboxylic acid (TCA) cycle and urea cycle. It actively participates in the transfer of metabolites that contribute to the synthesis of ATP linking important metabolic routes. The dicarboxylate carrier operates as a part of the family of mitochondrial transport proteins but does not form part of a larger protein complex.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe dicarboxylate carrier intersects several key metabolic pathways including the TCA cycle and gluconeogenesis. Through these pathways the protein collaborates with others like the adenine nucleotide translocase; it aids in maintaining energetic homeostasis within cells. The correct functioning of the dicarboxylate carrier ensures the proper flow of carbon skeletons essential for cellular respiration and energy production.\u003cbr\u003e\nThe malfunction of the mitochondrial dicarboxylate carrier links to metabolic conditions like renal tubular acidosis and certain forms of mitochondrial myopathy. The carrier's dysfunction may result in disrupted acid-base homeostasis within the kidneys due to impaired succinate transport. In these contexts proteins such as the mitochondrial pyruvate carrier may exhibit interconnected roles further influencing pathological states. Understanding the carrier's activity impacts strategies for addressing metabolic dysregulations associated with mitochondrial diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845148561577,"sku":"ab266122","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab266122","provider":"Iright","version":"1.0","type":"link"}