{"product_id":"abcam-ab266306","title":"Abcam, ab266306, Human NSD1 (KMT3B) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nNSD1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 22 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 22 bp deletion in exon 4\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NSD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nKMT3B also known as NSD1 is a histone methyltransferase enzyme with a significant role in chromatin modification. This protein adds methyl groups to histone H3 at lysine 36 (H3K36). With a molecular mass around 280 kDa NSD1 is mostly expressed in tissues such as the brain and skeletal muscle. It plays an integral role in controlling gene expression and maintaining genomic stability through its actions on chromatin.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNSD1 modulates transcription regulation by interacting with other nuclear proteins. As a part of the epigenetic regulation mechanism it is involved in complex processes of genome organization. By methylating H3K36 NSD1 affects chromatin dynamics and influences transcriptional activation and repression. NSD1 supports the orchestration of transcriptional changes in response to cellular signals and environmental stimuli.\u003cbr\u003e\nPathways\u003cbr\u003e\nNSD1 interfaces with transcriptional regulation and chromatin remodeling pathways. Its enzymatic action on H3K36 links NSD1 to pathways governing cell growth and differentiation. Through its interactions NSD1 associates with proteins like MLL and SETD2 highlighting its role in the regulation of gene expression and cellular developmental pathways.\u003cbr\u003e\nNSD1's dysfunction associates with Sotos syndrome and acute myeloid leukemia. Mutations in NSD1 lead to overgrowth and developmental delay characteristics observed in Sotos syndrome. In leukemia disrupted methylation patterns arise from NSD1 alterations affecting proteins like NUP98 contributing to oncogenic transformation and malignancy progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845163012265,"sku":"ab266306","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab266306","provider":"Iright","version":"1.0","type":"link"}