{"product_id":"abcam-ab266399","title":"Abcam, ab266399, Human ARPC5L knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nARPC5L KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 1 and Insertion of the selection cassette in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-ARPC5L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nARPC5L also known as Actin-Related Protein 2\/3 Complex Subunit 5-Like is a smaller subunit of the ARP2\/3 complex with a molecular mass around 16 kDa. This protein acts mechanically as an important regulator in the nucleation of new actin filaments aiding in the branching of the actin cytoskeleton. Scientists detect ARPC5L expression across diverse human tissues pointing to its broad role in cellular dynamics.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nARPC5L aids actin filament dynamics as part of the ARP2\/3 complex. This complex essential for cellular shape and motility helps drive the formation of lamellipodia and filopodian important structures in cell migration. Its function in remodeling the cytoskeleton makes it important in processes such as endocytosis and intracellular trafficking contributing to normal cell function and response to external stimuli.\u003cbr\u003e\nPathways\u003cbr\u003e\nARPC5L works within the cytoskeletal remodeling and cell motility pathways. Within the Wnt signaling pathway it plays a role in the regulation of cytoskeletal organization letting cells respond to signaling cues. The protein associates with others like ARPC1 and ARPC2 to facilitate these structural changes highlighting its importance to cellular adaptability and movement.\u003cbr\u003e\nARPC5L has connections to cancers including breast and colorectal cancer. Abnormal regulation or mutation of the ARP2\/3 complex can lead to altered cell migration and invasiveness contributing to cancer progression. The protein's interaction with elements of the Rho family such as RhoA and Rac1 integrates it into processes often hijacked in malignancies impacting how cells move invade and adhere.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845156032681,"sku":"ab266399","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab266399","provider":"Iright","version":"1.0","type":"link"}