{"product_id":"abcam-ab266440","title":"Abcam, ab266440, Human PDXK (PDXK.1) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPDXK KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 1 and Insertion of the selection cassette in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PDXK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPDXK.1 also known as pyridoxal kinase is an important enzyme that facilitates the phosphorylation of vitamin B6 to pyridoxal-5'-phosphate (PLP). Its molecular mass is approximately 35 kDa. You can find it expressed mainly in the liver kidney and brain tissues. PDXK.1 plays a role in converting vitamin B6 which is necessary for various enzymatic activities throughout the body.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPDXK.1 functions within cellular environments by catalyzing the formation of PLP an active form of vitamin B6. PLP acts as a coenzyme involved in numerous reactions such as amino acid metabolism neurotransmitter synthesis and modulation of hormone activity. PDXK.1 does not work in isolation but instead interacts with other proteins in these processes though it is not a part of a larger complex.\u003cbr\u003e\nPathways\u003cbr\u003e\nPDXK.1 is important for the vitamin B6 metabolism pathway. This pathway integrates with networks involving amino acid catabolism and neurotransmitter pathways essential for metabolic functions and nervous system regulation. An association exists between PDXK.1 and proteins like cysteine desulfurase which also rely on PLP for their enzymatic activities highlighting its involvement in broader biochemical systems.\u003cbr\u003e\nPDXK.1 has been linked with metabolic disorders and neurological conditions. A deficiency or dysfunction in PDXK.1 activity can contribute to conditions such as pyridoxine-dependent epilepsy and some forms of anemia. In these cases disruptions in the metabolism of vitamin B6 due to inadequate levels of PLP may directly affect proteins like glutamate decarboxylase which require PLP for proper function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845157310633,"sku":"ab266440","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab266440","provider":"Iright","version":"1.0","type":"link"}