{"product_id":"abcam-ab266695","title":"Abcam, ab266695, Human PEA15 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nPEA15 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 14 bp deletion in exon 2\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PEA15, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPEA15 also known as phosphoprotein enriched in astrocytes 15 or PED is a multifunctional protein weighing around 15 kDa. Mechanically PEA15 acts as a death effector domain (DED)-containing protein that modulates diverse cellular processes by binding to and inhibiting other DED-containing proteins. This protein shows wide expression notably in the central nervous system including the brain where it exerts critical roles in signaling networks.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPEA15 plays pivotal roles in promoting cellular survival and regulating apoptosis within various cell types. It operates by modulating pathways like apoptosis and cell proliferation often forming complexes with other DED-containing proteins such as FADD and caspase-8. By interacting with these proteins PEA15 can maintain cellular stability and manage stress responses illustrating its significant contribution to cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nPEA15 is integral in mediating the extracellular signal-regulated kinase (ERK) and MAP kinase pathways. As PEA15 binds to ERK1\/2 it retains these kinases in the cytoplasm impeding their translocation to the nucleus and therefore affecting the transcription of target genes. Furthermore its interactions with Ras-related proteins indicate its involvement in the regulation of cell growth and differentiation showing its broad influence on cellular signaling cascades.\u003cbr\u003e\nPEA15 correlates with neurological conditions including Alzheimer's disease and gliomas. In Alzheimer's disease PEA15 modulates neuroinflammation and amyloid precursor protein processing collaborating with proteins such as tau and amyloid-beta. In gliomas alterations in PEA15 expression influence tumor progression and treatment susceptibility highlighting important intersections with oncogenic factors like EGFR and PTEN. These associations spotlight PEA15 as a potential therapeutic target in both neurodegenerative and cancerous disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845196894377,"sku":"ab266695","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab266695","provider":"Iright","version":"1.0","type":"link"}