{"product_id":"abcam-ab266757","title":"Abcam, ab266757, Human NDUFA4 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nNDUFA4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 31 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 31 bp deletion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NDUFA4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNDUFA4 also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 is a small protein with a molecular mass of around 9 kDa. It plays a role as part of the mitochondrial respiratory chain where it contributes to the electron transport process. NDUFA4 is expressed in various tissues but has higher levels of expression in tissues with elevated metabolic demand like the heart liver and muscle. Its location in the inner mitochondrial membrane allows it to participate directly in respiration activity by aiding electron transfer from NADH to ubiquinone.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNDUFA4 functions as part of the larger protein complex known as Complex I or NADH:ubiquinone oxidoreductase. Complex I is a critical component of the mitochondrial electron transport chain. It supports ATP synthesis by creating a proton gradient across the inner mitochondrial membrane. NDUFA4 participates in this energy conversion process and cooperates with additional protein subunits in Complex I to maintain cellular energy metabolism. This makes it essential for maintaining efficient aerobic respiration.\u003cbr\u003e\nPathways\u003cbr\u003e\nNDUFA4 has a significant role within the oxidative phosphorylation pathway a central metabolic pathway in cellular energy production. Within this pathway it associates with other proteins such as NDUFB8 and NDUFS1 in Complex I which work together for effective electron transport. NDUFA4 also intersects with the broader pathways of metabolic regulation lending support to the processes that produce ATP as a cellular energy currency.\u003cbr\u003e\nMutations or dysregulation of NDUFA4 have been connected to mitochondrial diseases such as mitochondrial myopathy. These conditions arise due to impaired energy production in cells. Additionally alterations in NDUFA4 levels or activity have implications in neurodegenerative disorders like Parkinson's disease. In such diseases proteins like NDUFS4 another Complex I subunit also become affected highlighting potential protein-protein interactions critical for maintaining mitochondrial health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845196763305,"sku":"ab266757","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab266757","provider":"Iright","version":"1.0","type":"link"}