{"product_id":"abcam-ab266868","title":"Abcam, ab266868, Human MLST8 (G protein beta subunit like\/GBL) knockout HCT116 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nMLST8 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HCT116,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Colon,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 2,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-MLST8, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe G protein beta subunit-like protein often known as GBL is an essential component in cellular signaling. It plays a mechanical role as part of the larger G protein complex that transduces signals from cell surface receptors to intracellular pathways. Though we do not have a precise molecular weight for GBL it is known to interact closely with other subunits of the G protein complex. GBL is widely expressed across various tissues reflecting its involvement in numerous cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGBL contributes to the regulation and setting up of signal transduction pathways by forming a part of the G protein complex. This complex involves the alpha beta and gamma subunits where GBL typically interacts with the beta subunit family. By binding to receptors like GPCRs GBL facilitates the exchange of GDP for GTP on the G protein alpha subunit thereby activating downstream effectors. The complex is critical in ensuring precise cellular responses and GBL maintains structural integrity within it.\u003cbr\u003e\nPathways\u003cbr\u003e\nGBL plays an integral role in the G protein-coupled receptor (GPCR) signaling and the MAPK signaling pathways. In GPCR signaling GBL's interaction with the beta subunit influences processes like sensory perception and cell growth. In the MAPK pathway critical for cell responses to growth signals GBL connects with proteins such as RAF and MEK supporting the cascade of phosphorylation events critical for pathway progression. These pathways highlight GBL's importance in cellular dynamics and network interactions.\u003cbr\u003e\nScientists associate GBL with certain cancers and metabolic disorders. Aberrant expression or dysfunction of GBL can lead to disrupted signal transduction an important feature in pathways involved in tumorigenesis such as those involving GPCR signaling anomalies. Additionally the MAPK pathway's misregulation where GBL plays a part links to metabolic disorders. This makes GBL a potential therapeutic target offering insights into developing treatments by targeting associated proteins like GPCRs and components of the MAPK pathway.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845205282985,"sku":"ab266868","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab266868","provider":"Iright","version":"1.0","type":"link"}