{"product_id":"abcam-ab267039","title":"Abcam, ab267039, Human SAMD9 knockout A549 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nSAMD9 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 2 and 2 bp deletion in exon 2,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SAMD9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSAMD9 also known as sterile alpha motif domain-containing 9 is an intriguing protein with a molecular mass of approximately 180 kDa. Predominantly expressed in tissues such as bone marrow and lymph nodes it plays a role in cellular proliferation and response to stress stimuli. The protein is part of the larger family of SAMD proteins which contain sterile alpha motifs influencing protein-protein interactions and it engages in regulation steps at a molecular level.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nSAMD9 takes part in controlling cellular proliferation and inhibiting abnormal growth mostly acting as a growth suppressor. Its functions link to maintaining homeostasis within cells and aiding in preventing uncontrolled cell expansion. SAMD9 functions independently of any known stable protein complex performing its regulatory role in diverse cellular contexts. The protein's involvement spans multiple cell types reflecting its broad impact on normal human physiology.\u003cbr\u003e\nPathways\u003cbr\u003e\nSAMD9 interacts with critical signaling modules such as the mTOR pathway and the interferon signaling pathway. It modulates cellular responses related to growth control and stress adaptation. Interactions with other proteins like TSC1 and TSC2 within the mTOR pathway highlight SAMD9's engagement in regulating cellular responses to environmental changes and nutrient availability.\u003cbr\u003e\nSAMD9 is associated with severe conditions like myelodysplastic syndromes and MIRAGE syndrome. These conditions link to mutations affecting the protein's regulatory capacities leading to disrupted cellular processes and disease progression. The protein shares connections with SAMD9L another related protein indicating a potential overlap in disease mechanisms and impacts. Understanding the behavior of SAMD9 in these contexts is important for developing therapeutic strategies and improving disease management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845066608809,"sku":"ab267039","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab267039","provider":"Iright","version":"1.0","type":"link"}