{"product_id":"abcam-ab267115","title":"Abcam, ab267115, Human RTP4 knockout A549 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nRTP4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 5 bp deletion in exon 2,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RTP4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRTP4 also known as Receptor Transporter Protein 4 plays a significant role in the endoplasmic reticulum where it facilitates the trafficking and proper function of olfactory receptors. It has a molecular mass of approximately 31 kDa. RTP4 expression is mainly found in the olfactory sensory neurons but also in various other tissues such as the brain and liver. It helps in guiding G protein-coupled receptors (GPCRs) to the cell membrane ensuring their functional expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe RTP4 protein aids in the transport and functional expression of GPCRs without forming part of any known stable complexes. This chaperone activity is vital for GPCR function impacting signal transduction by promoting proper receptor folding and surface expression. RTP4 ensures that olfactory and other related receptors can adequately respond to stimuli. Its chaperone activity is important for maintaining GPCR dynamics in various cell types.\u003cbr\u003e\nPathways\u003cbr\u003e\nRTP4 significantly influences GPCR signaling pathways and olfactory signaling pathways. It directly affects the transport and activation of olfactory receptors key components in the olfactory signaling pathway. RTP4's interaction with olfactory receptors impacts the downstream components such as adenylyl cyclase leading to successful signal transduction. Additionally RTP4 interacts with proteins in GPCR signaling that modify receptor availability on the cell surface.\u003cbr\u003e\nRTP4 has implications in anosmia the loss or impairment of the sense of smell and potentially in liver-related disorders. Disruption of RTP4 function can lead to defective olfactory receptor trafficking resulting in anosmia. RTP4-related pathways implicate proteins like OR51E1 in olfactory dysfunctions. There may be a less clear association with liver disorders involving misfolded GPCRs; however this link requires further exploration.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845203349673,"sku":"ab267115","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab267115","provider":"Iright","version":"1.0","type":"link"}