{"product_id":"abcam-ab267312","title":"Abcam, ab267312, Human FUK knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nFCSK KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 11 and 1 bp deletion in exon 11 and 25 bp deletion in exon 11. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 10 bp deletion in exon 11 and 1 bp deletion in exon 11 and 25 bp deletion in exon 11\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-FCSK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein FUK also known as fucokinase functions mechanistically as an enzyme that catalyzes the phosphorylation of L-fucose a monosaccharide involved in various metabolic processes. The molecular mass of FUK is approximately 131 kDa. FUK is primarily expressed in the liver but also in other tissues like the kidney and brain where it performs its enzymatic role as part of cellular metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFUK plays a role in the salvage pathway of L-fucose metabolism. This pathway recycles free L-fucose into GDP-fucose which becomes essential for glycoprotein and glycolipid synthesis. FUK operates not as part of a larger protein complex but individually facilitating the utilization of fucose derived from dietary sources or breakdown of glycoproteins. The enzyme regulates intracellular concentrations of fucose impacting cell surface glycoconjugates important for cell signaling.\u003cbr\u003e\nPathways\u003cbr\u003e\nFUK integrates into the fucose salvage pathway that links to glycosylation processes. This situates FUK within the larger context of glycan biosynthesis pathways where it intersects with enzymes such as GDP-fucose synthase. These pathways significantly influence the modification and function of proteins and lipids on cell surfaces affecting cellular communication and host-pathogen interactions.\u003cbr\u003e\nFUK has connections to congenital disorders of glycosylation and cancer. Deficiencies in FUK enzyme activity can lead to disrupted fucosylation which contributes to clinical features seen in glycosylation disorders. In cancer abnormal fucose metabolism affects tumor progression and metastasis involving interactions with proteins such as fucosyltransferases which modify glycans on cell surfaces. Understanding FUK's role could provide insights into therapeutic approaches for glycol-related diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845152690345,"sku":"ab267312","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab267312","provider":"Iright","version":"1.0","type":"link"}