{"product_id":"abcam-ab267332","title":"Abcam, ab267332, Human JOSD1 knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nJOSD1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-JOSD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nJOSD1 also known as Josephin domain-containing protein 1 functions as a deubiquitinating enzyme. It specifically removes ubiquitin from protein substrates which regulates protein degradation localization and activity in cells. The protein mass is approximately 45 kDa. JOSD1 is expressed in various tissues with significant levels seen in the brain liver and kidney. By modulating the ubiquitination status of substrates JOSD1 influences cellular processes like signal transduction and response to stress.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein modifies the stability and activity of other proteins by removing ubiquitin molecules. It acts as part of a larger deubiquitinase complex which interacts with protein networks to maintain cellular homeostasis. Through its enzyme activity JOSD1 contributes to the regulation of processes such as protein quality control and cell cycle progression.\u003cbr\u003e\nPathways\u003cbr\u003e\nScientists have placed JOSD1 in the ubiquitin-proteasome system an important pathway responsible for protein turnover in cells. It has connections with other proteins like USP7 and USP9X in this pathway reflecting its coordination role in protein deubiquitination. JOSD1 also participates in the endoplasmic reticulum-associated degradation (ERAD) pathway helping to eliminate misfolded proteins important for maintaining protein folding homeostasis within the cell.\u003cbr\u003e\nAbnormal activity of JOSD1 can connect to neurodegenerative disorders such as Alzheimer's disease where protein misfolding and aggregation play significant roles. JOSD1's interaction with proteins like tau and amyloid precursor protein suggests its potential to influence disease progression. Additionally research associates JOSD1 with liver cancer where dysregulated ubiquitin signaling may contribute to tumor development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845198434473,"sku":"ab267332","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab267332","provider":"Iright","version":"1.0","type":"link"}