{"product_id":"abcam-ab267568","title":"Abcam, ab267568, Human NrCAM ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman NrCAM ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human NrCAM in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture media, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.3 ng\/mL,\u003cbr\u003e\nRange:0.328 - 80 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman NrCAM ELISA Kit is designed for the quantitative determination of NRCAM in cell culture supernatants, plasma and serum samples.\u003cbr\u003e\nΔNote:\u003cbr\u003e\nHuman NrCAM concentration is low in normal serum and plasma samples and may not be detectable in this assay.\u003cbr\u003e\nThis assay employs an antibody specific for human NrCAM coated on a 96-well plate. Standards and samples are pipetted into the wells and NrCAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human NrCAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NrCAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nNrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.\u003cbr\u003e\nNrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843775058089,"sku":"ab267568","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab267568","provider":"Iright","version":"1.0","type":"link"}