{"product_id":"abcam-ab267581","title":"Abcam, ab267581, Mouse Neprilysin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse Neprilysin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Neprilysin in Mouse in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture media, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 61 pg\/mL,\u003cbr\u003e\nRange:61.44 - 15000 pg\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Neprilysin ELISA Kit is designed for the quantitative determination of Neprilysin in cell culture supernatants, plasma and serum samples.\u003cbr\u003e\nThis assay employs an antibody specific for mouse Neprilysin coated on a 96-well plate. Standards and samples are pipetted into the wells and Neprilysin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Neprilysin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neprilysin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nThis ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40\/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP1alpha, MIP-1gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.\u003cbr\u003e\nPathways\u003cbr\u003e\nNeprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.\u003cbr\u003e\nCD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843765653673,"sku":"ab267581","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab267581","provider":"Iright","version":"1.0","type":"link"}