{"product_id":"abcam-ab268748","title":"Abcam, ab268748, Recombinant human GWL protein (Active)","description":"\u003cp\u003eSize: 5µg\u003cbr\u003e\nRecombinant human GWL protein (Active) is a Human Full Length protein, in the 1 to 879 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;70%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;70% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The specific activity was determined to be 16.2 nmol\/min\/mg in a kinase assay using ARPP19 protein substrate.,\u003cbr\u003e\nAccession:Q96GX5,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGWL also known as Greatwall kinase (and occasionally Mastl) is a protein kinase with a calculated molecular mass of about 58 kDa. This protein mainly expresses in various tissues but it shows higher expression in organs such as the heart liver and skeletal muscle. GWL plays a mechanical role in the regulation of cell cycle through its kinase activity which phosphorylates other key proteins involved in mitosis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGWL influences the cell cycle and mitotic progression by modulating the activity of the phosphatase inhibitor proteins ENSA and ARPP19. It is part of a functional complex with these proteins when activated leading to the inhibition of Protein Phosphatase 2A (PP2A) during mitosis which allows for proper chromosome condensation and segregation. This ensures that the cells divide accurately maintaining genomic stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nDifferent cellular pathways integrate their signals through GWL to regulate mitosis. One of the key pathways is the cell cycle control pathway where GWL works alongside CDK1 and is essential for the phosphorylation cascade that leads to mitotic entry. Another pathway of note is the mitotic spindle assembly checkpoint where GWL interacts with proteins such as BUB1 and MAD2 helping establish mechanisms that ensure cells do not prematurely progress through mitosis with errors.\u003cbr\u003e\nGWL dysfunction links to cancer due to its critical role in cell division. Aberrant expression or mutation of GWL can lead to aneuploidy and genomic instability factors that contribute to tumorigenesis. Moreover abnormalities in GWL expression have been associated with neurological disorders like Alzheimer's disease where it may interact with tau protein aggregation pathways. In cancer GWL often works in conjunction with oncogenes like MYC and tumor suppressors such as p53 influencing their roles in controlling cell proliferation and survival.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843978809513,"sku":"ab268748","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab268748","provider":"Iright","version":"1.0","type":"link"}