{"product_id":"abcam-ab268853","title":"Abcam, ab268853, Recombinant human PI 3 Kinase p85 alpha + PI 3 Kinase catalytic subunit alpha\/PIK3CA protein","description":"\u003cp\u003eSize: 5µg\u003cbr\u003e\nRecombinant human PI 3 Kinase p85 alpha + PI 3 Kinase catalytic subunit alpha\/PIK3CA protein is a Human Full Length protein, in the 1 to 1068 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;70%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;70% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The specific activity of ab268853 was 1,200 nmol\/min\/mg in a kinase assay using a PI(4,5)P2:PS mixture as substrate.,\u003cbr\u003e\nAccession:P42336,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nSubunits co-expressed.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe PI 3 Kinase p85 alpha also known as PIK3R1 and PI 3 Kinase catalytic subunit alpha (PIK3CA) functions as an important component in cellular signal transduction. These proteins form a Class IA PI 3-kinase complex where p85 alpha serves as the regulatory subunit and p110 alpha acts as the catalytic subunit. PIK3CA the catalytic subunit weighs approximately 124 kDa. These proteins are expressed in various tissues with high expression in adipose tissue liver and the brain playing a significant role in mediating cell growth proliferation and metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThese proteins operate as part of a lipid kinase complex. They phosphorylate the inositol ring of phosphoinositides at the 3-position important in the generation of lipid second messengers. The active PI 3-kinase complex primarily phosphorylates phosphatidylinositol (PI) phosphatidylinositol 45-bisphosphate (PI(45)P2) creating phosphatidylinositol 345-trisphosphate (PI(345)P3) which then propagates intracellular signaling cascades. This activity regulates various cellular processes including survival and migration.\u003cbr\u003e\nPathways\u003cbr\u003e\nPI 3 Kinase p85 alpha and PIK3CA are integral to the PI3K\/AKT\/mTOR signaling pathway. This pathway plays a pivotal role in regulating cell cycle and is intricately involved in pathways that control cellular quiescence proliferation cancer and longevity. The pathway's components such as AKT and mTOR are critical effectors of PI3K signaling. Additionally the proteins interrelate with other pathways through interactions with proteins like PTEN which acts as a negative regulator by dephosphorylating PI(345)P3.\u003cbr\u003e\nThese components have significant associations with oncogenic processes due to mutations in PIK3CA that are frequently observed in various cancers including breast and colorectal cancer. Such mutations lead to constitutive activation of the PI3K\/AKT pathway promoting tumorigenesis. The proteins also link to insulin resistance-related disorders as enhanced PI3K activity affects metabolic signaling. Disorders like these connect through interactions with proteins such as IRS-1 which becomes dysregulated in the context of these diseases further highlighting their implication in aberrant signaling pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843923071145,"sku":"ab268853","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab268853","provider":"Iright","version":"1.0","type":"link"}