{"product_id":"abcam-ab270023","title":"Abcam, ab270023, Maleimide-R Phycoerythrin","description":"\u003cp\u003eSize: 2mg\u003cbr\u003e\nMaleimide-R Phycoerythrin (ab270023) is part of the biochemicals range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nForm:LyophilizedSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMaleimide-Activated R Phycoerythrin (ab270023) is designed to facilitate the conjugation of R-Phycoerythrin to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to R-Phycoerythrin allows conjugation to be performed very efficiently at physiological pH.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company. It was previously called Maleimide-R Phycoerythrin (2mg)SKU: 403-0002. Expedeon product code 403-0002 is the same as the 2 mg size of this product.\u003cbr\u003e\nThe buffer in which the thiolated molecule is dissolved should be in the pH range 6.5-7.5. Phosphate buffered saline pH 7.2 is commonly employed. EDTA (1-10 mM) may help to prevent metal catalyzed oxidation of thiols.\u003cbr\u003e\nPlease see the below table for recommended buffer conditions and components:\u003cbr\u003e\nBuffer components\u003cbr\u003e\nCompatible\u003cbr\u003e\npH range 6.5-7.5\u003cbr\u003e\nChelating agents (e.g. EDTA)\u003cbr\u003e\nPrimary amines\u003cbr\u003e\nReducing reagents\u003cbr\u003e\nSodium Azide\u003cbr\u003e\nMolecules that do not contain –SH groups can be chemically modified to introduce the required thiol functionality.\u003cbr\u003e\nAbcam offers a thiolation kit (ab270020) compatible with this product.\u003cbr\u003e\nIn the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols. Any reducing reagent (e. g. dithiothreitol and β-mercaptoethanol) used to produce free thiols should be removed before proceeding with the conjugation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847443927209,"sku":"ab270023","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab270023","provider":"Iright","version":"1.0","type":"link"}