{"product_id":"abcam-ab270048","title":"Abcam, ab270048, His-Tag Protein Expression Check Kit","description":"\u003cp\u003eSize: 30Test\u003cbr\u003e\nHis-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nHis-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA. The assay provides a qualitative yes\/no answer, although a semi-quantitative measurement of the protein abundance in the sample can be determined using internal references and controls.\u003cbr\u003e\nA His-tagged protein on the \"Test line\" (T line) and a ULFA-tagged protein on the \"Control line\" (C line) are immobilized on the nitrocellulose strips.\u003cbr\u003e\n40nm Gold conjugated anti-HisTag antibody and 40 nm Gold conjugated anti-ULFA tag conjugates are dried on the conjugate pad and will selectively bind to the T and C line, respectively. The C line is assay-independent and should always appear as a strong red line; if it is not visible, the test is not valid and should be repeated.\u003cbr\u003e\nHis-Tag Protein Expression Check Kit is formulated as a competitive assay so that when the sample does not contain any His-tagged protein, the T line will appear as a strong red line. On the contrary, the T line will not be visible (or appear as a pale red line) if the sample contains the His- tagged protein that competes with the binding of the Gold conjugated anti-HisTag antibody conjugate to the antigen immobilized on the T line.\u003cbr\u003e\nBenefits:\u003cbr\u003e\nQuick and easy qualitative detection of His-tagged proteins\u003cbr\u003e\nCompatible with cell culture media and lysate\u003cbr\u003e\nNo costly equipment required\u003cbr\u003e\nSmall sample volumes required (80 μL)\u003cbr\u003e\nCompatible with Ni-NTA Affinity Resin - Amintra® (\u003cbr\u003e\nab270549\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company. It was previously called His-Tag Check\u0026amp;Go!. Expedeon product code 4003-0030 is the same as the 30-test size of this product.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847597740201,"sku":"ab270048","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab270048","provider":"Iright","version":"1.0","type":"link"}