{"product_id":"abcam-ab270062","title":"Abcam, ab270062, Recombinant human IGF1 protein (Active)","description":"\u003cp\u003eSize: 50µg \/ 100µg \/ 250µg\u003cbr\u003e\nRecombinant human IGF1 protein (Active) is a Human Full Length protein in the 49 to 118 aa range with ≥95% purity, \u0026lt; 0.005 EU\/µg endotoxin level and suitable for SDS-PAGE, Functional studies, Cell Culture, mass spectrometry and HPLC. The predicted molecular weight of ab270062 protein is 7.7 kDa.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 0.005 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:Cell Culture, HPLC, SDS-PAGE, Mass Spec, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells.ED50 is ≤ 0.9 ng\/mL, corresponding to a specific activity of 1.11 x 106 units\/mg.,\u003cbr\u003e\nAccession:P05019,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in PBS,\u003cbr\u003e\nStorage buffer:pH: 6 - 8Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our recombinant human IGF1 protein ab270062 as a control.\u003cbr\u003e\nThe ab270062 IGF-1 protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nPremium Bioactive Protein range\u003cbr\u003e\nThe ab270062 IGF-1 protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.\u003cbr\u003e\nMore premium bioactive proteins can be found\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.\u003cbr\u003e\nPathways\u003cbr\u003e\nIGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.\u003cbr\u003e\nAlterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843798192297,"sku":"ab270062","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab270062","provider":"Iright","version":"1.0","type":"link"}