{"product_id":"abcam-ab270449","title":"Abcam, ab270449, Anti-NLRP3 antibody [EPR23073-96]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-NLRP3 antibody [EPR23073-96] (ab270449) is a rabbit monoclonal antibody detecting NLRP3 in  Western Blot, IP, ICC\/IF . Suitable for  Mouse . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR23073-96,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nApplications:IP, WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:NLRP3 is related with inflammation and is cell-type dependent. This antibody detects no signal or very weak signal in most normal natural samples. Stimulation may be required to allow detection of the NLRP3 protein in most samples, as described in the literature (PMID: 29070054, 29434227, and 22569257).\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-NLRP3 antibody [EPR23073-96] (ab270449) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Mouse samples.\u003cbr\u003e\nWhat is the molecular weight of NLRP3?\u003cbr\u003e\nAnti-NLRP3 [EPR23073-96] (ab270449) specifically detects a band for NLRP3 (UniProt: Q8R4B8) at a molecular weight of 118kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-NLRP3 [EPR23073-96] (ab270449) was first used in a scientific publication in 2020 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-NLRP3 antibody [EPR23073-96] (ab270449) has been confirmed by Western blot testing in Nlrp3 Knockout Mouse BMDCs.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR23073-96] also available for your convenience: ab270449, Carrier free -\u003cbr\u003e\nab272702\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNLRP3 also known as NALP3 or cryopyrin is a protein important for the inflammatory response. It is a component of the inflammasome which senses pathogenic microorganisms and stress. NLRP3 weighs around 118 kDa and is widely expressed in innate immune cells particularly macrophages and dendritic cells. Researchers often use techniques such as 'NLRP3 WB' (Western blot) and 'NLRP3 immunofluorescence' to study NLRP3 expression and localization.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNLRP3 is central to the body's defense mechanism by participating in forming the inflammasome complex which activates caspase-1. This activation leads to the cleavage of pro-inflammatory cytokines like IL-1Β and IL-18 into their active forms. NLRP3's function is essential for mounting an effective immune response to infections and damage signals. It senses a range of stimuli including crystalline substances and cellular stress triggering an immune response. Its expression is tightly regulated to prevent excessive inflammation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe NLRP3 inflammasome is key in the innate immune response and inflammation pathways. It links cellular stress signals to inflammation through the activation of caspase-1 which is important for the IL-1 signaling pathway. NLRP3 interacts with other proteins like ASC (apoptosis-associated speck-like protein containing a CARD) to form the inflammasome complex which is a core part of the inflammatory signaling pathways and links innate and adaptive immunity.\u003cbr\u003e\nNLRP3 has a significant role in inflammatory diseases and is linked to conditions like cryopyrin-associated periodic syndromes (CAPS) and gout. Mutations or dysregulation of NLRP3 lead to hyperactivation resulting in autoimmune and autoinflammatory conditions. NLRP3's interaction with IL-1 contributes to the excessive inflammatory response seen in these disorders. Understanding NLRP3's function in these pathways can inform therapeutic approaches targeting chronic inflammatory diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844040052905,"sku":"ab270449","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab270449","provider":"Iright","version":"1.0","type":"link"}