{"product_id":"abcam-ab270778","title":"Abcam, ab270778, Serine Protease Assay Kit (FAM-Spacer-Leu-CMK)","description":"\u003cp\u003eSize: 25Test \/ 100Test\u003cbr\u003e\nThese Serine Protease Assay Kits enable researchers to detect intracellular chymotrypsin-like serine protease activity  in vitro  without lysing the cell.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nThese Serine Protease Assay Kits enable researchers to detect intracellular chymotrypsin-like serine protease activity\u003cbr\u003e\nin vitro\u003cbr\u003e\nwithout lysing the cell.\u003cbr\u003e\nFLISP® (Fluorescent Labeled Inhibitors of Serine Proteases) reagents are non-cytotoxic green or red fluorescent inhibitors that covalently bind with active serine protease enzymes. FLISP is a powerful method to assess the intracellular levels of chymotrypsin- like serine protease activity in vitro. Just add FLISP directly to the cell culture media, incubate, and wash. Because FLISP inhibitors are cell-permeant, they will efficiently diffuse in and out of all living cells. If there is an active chymotrypsin-like enzyme inside the cell, it will covalently bind with the FLISP inhibitor and retain the green or red fluorescent signal within the cell. Cells containing lower concentrations of chymotrypsin-like enzyme activity will retain a lower level of fluorescence compared to cells containing higher concentrations of this effector enzyme. There is no interference from pro-enzymes nor inactive forms of the enzymes. If the treatment is activating chymotrypsin-like serine proteases, positive cells will fluoresce brighter than the normal baseline negative cells, thus enabling researchers to clearly differentiate the populations.\u003cbr\u003e\nProduced using a non-baculovirus expression system.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847697813673,"sku":"ab270778","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab270778","provider":"Iright","version":"1.0","type":"link"}