{"product_id":"abcam-ab272198","title":"Abcam, ab272198, Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) is a single-wash 90-min Simplestep used to quantify Human Pro-Collagen I C-Terminal Propeptide (PICP) with a sensitivity of 8.15 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 8.15 pg\/mL,\u003cbr\u003e\nRange:23.44 - 1500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) ab272198 is a rapid single-wash 90-min Sandwich ELISA to measure Human Pro-Collagen I C-Terminal Propeptide (PICP)in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 8.15 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Pro-Collagen I C-Terminal Propeptide (PICP)SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) ab272198 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-PICP antibody [EPR22894-16] - BSA and Azide free (Capture)\u003cbr\u003e\nab272379\u003cbr\u003e\n- Anti-PICP antibody [EPR22209-103] - BSA and Azide free (Detector)\u003cbr\u003e\nab272379\u003cbr\u003e\nPro-Collagen I C-Terminal Propeptide (PICP), encoded by the\u003cbr\u003e\nCOL1A1\u003cbr\u003e\ngene, is the fibrillar collagen that serves as a major component of type I collagen found in cartilage and most connective tissues. Studies have shown that mutations in the mutations in the Pro-Collagen I C-Terminal Propeptide are associated with osteoporosis, osteogenesis imperfecta diseases, and Ehlers-Danlos syndrome. The standard protein in this product is the C‑terminal Propeptide region of Collagen alpha-1(I).\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPICP also known as procollagen type 1 C-peptide or collagen C-peptide is a byproduct released during the synthesis of type I collagen. Type I collagen is the most abundant collagen type in the human body providing structure to skin bone tendons and other connective tissues. PICP has a molecular mass of approximately 35-38 kDa. It is expressed in tissues where type I collagen is synthesized including bone and skin fibroblasts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPICP serves as a marker for collagen biosynthesis closely reflecting the rate of collagen type I production. PICP does not form part of a larger complex but exists as a separate entity once cleaved from procollagen. Its presence in the bloodstream allows researchers to monitor collagen turnover which is vital in understanding tissue formation and remodeling processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nPICP connects with collagen synthesis and deposition pathways particularly relevant in bone formation and skin repair. It interacts indirectly with proteins like collagenase during collagen degradation and remodeling. Increased PICP levels indicate heightened collagen synthesis correlating with anabolic processes especially in bone metabolism pathways.\u003cbr\u003e\nPICP levels are significant in bone-related conditions like osteoporosis where decreased collagen synthesis is often observed. It also correlates with fibrotic conditions including liver fibrosis where elevated collagen production marks disease progression. In these contexts PICP relates to other proteins such as procollagen type III N-peptide (PIIINP) which also serve as markers of pathological collagen deposition.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843574091945,"sku":"ab272198","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab272198","provider":"Iright","version":"1.0","type":"link"}