{"product_id":"abcam-ab272203","title":"Abcam, ab272203, Human TGFB3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman TGFB3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human TGFB3 with a sensitivity of 0.038 ng\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Extracts, Cell culture media,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.038 ng\/mL,\u003cbr\u003e\nRange:0.38 - 24 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman TGFB3 ELISA Kit ab272203 is a rapid single-wash 90-min Sandwich ELISA to measure Human TGFB3 in cell culture media, tissue extracts. This SimpleStep sensitivity is 0.038 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman TGFB3 SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman TGFB3 ELISA Kit ab272203 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-TGF beta 3 antibody [EPR21888-209] - BSA and Azide free (Capture)\u003cbr\u003e\nab272387\u003cbr\u003e\n- Anti-TGF beta 3 antibody [EPR21888-220] - BSA and Azide free (Detector)\u003cbr\u003e\nab272387\u003cbr\u003e\nTransforming growth factor beta-3 is a cytokine encoded by the TGFB3 gene. TGFB3 belongs to a larger family of proteins known as the transforming growth factor beta superfamily, which includes TGFB1 and 2, bone morphogenetic proteins, Growth Differentiation factors, inhibins and activins. TGFB3 is expressed as a proprotein containing a 277- amino acid latency-associated peptide (LAP) that remains non-covalently associated with TGFB3 after cleavage by the Golgi apparatus. Interaction with integrins removes the LAP, thus releasing a 112- amino acid active TGFB3. Active TGFB3 has many functions, including regulation of the embryogenesis and secondary palate development cell differentiation. The standard protein in this product is the active sequence of TGFB3.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843537850537,"sku":"ab272203","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab272203","provider":"Iright","version":"1.0","type":"link"}