{"product_id":"abcam-ab272206","title":"Abcam, ab272206, Human DPT ELISA Kit (TRAMP)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman DPT ELISA Kit (TRAMP) is a single-wash 90-min Simplestep used to quantify Human DPT (TRAMP) with a sensitivity of 2.94 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Citrate plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.94 pg\/mL,\u003cbr\u003e\nRange:18.75 - 1200 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman DPT ELISA Kit (TRAMP) ab272206 is a rapid single-wash 90-min Sandwich ELISA to measure Human DPT (TRAMP)in cell culture media, citrate plasma, serum. This SimpleStep sensitivity is 2.94 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman DPT (TRAMP)SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman DPT ELISA Kit (TRAMP) ab272206 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-DPT\/TRAMP antibody [EPR22845-33] - BSA and Azide free (Capture)\u003cbr\u003e\nab272411\u003cbr\u003e\n- Anti-DPT\/TRAMP antibody [EPR22845-41] - BSA and Azide free (Detector)\u003cbr\u003e\nab272411\u003cbr\u003e\nDPT (TRAMP or Dermatopontin) is a matrix protein found in the intracellular junction of the extracellular matrix. DPT is increased in myocardial infarction and decreased in fibrosis and leiomyoma. DPT is a biomarker for giant-cell tumor of the bone and oral squamous cell carcinoma.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDPT also known as Dermatopontin is a protein with a molecular mass of approximately 22 kDa. It is expressed in various tissues with high expression levels in the skin heart and lungs. Dermatopontin interacts with extracellular matrix components and influences cell adhesion and matrix organization. The protein plays a role in regulating fibroblast activity and collagen binding making it important in connective tissue structure and function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDermatopontin contributes to maintaining dermal structural integrity and facilitating wound healing. As part of a larger protein interaction network it enhances cellular communication and tissue repair processes. Dermatopontin modulates the bioavailability of TGF-beta which is essential for collagen production and tissue homeostasis. This highlights its presence in skin remodeling and repair mechanisms linking it to new tissue formation.\u003cbr\u003e\nPathways\u003cbr\u003e\nDermatopontin participates actively in the TGF-beta signaling pathway and the ECM-receptor interaction pathway. These pathways are critical for cellular communication and tissue structuring processes. The protein interacts with Integrins and Fibronectin facilitating cell-to-ECM adhesion and remodeling activities. Consequently Dermatopontin's pathway involvement supports dynamic tissue responses such as healing and functional adaptation to environmental changes.\u003cbr\u003e\nDermatopontin shows associations with fibrosis and keloids. These disorders feature aberrant tissue formation and scarring wherein DPT's regulation of fibroblasts and collagen dynamics plays a notable role. Additionally Dermatopontin's interactions with other proteins like Collagen VI and Decorin influence its involvement in these conditions contributing to the understanding of pathological tissue remodeling mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843568980137,"sku":"ab272206","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab272206","provider":"Iright","version":"1.0","type":"link"}