{"product_id":"abcam-ab272470","title":"Abcam, ab272470, Human BMP9 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman BMP9 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human BMP9 with a sensitivity of 0.97 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Citrate plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.97 pg\/mL,\u003cbr\u003e\nRange:4.69 - 300 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman BMP9 ELISA Kit ab272470 is a rapid single-wash 90-min Sandwich ELISA to measure Human BMP9 in cell culture supernatant, citrate plasma, serum. This SimpleStep sensitivity is 0.97 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman BMP9 SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman BMP9 ELISA Kit ab272470 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-BMP9 antibody [EPR22898-235] - BSA and Azide free (Capture)\u003cbr\u003e\nab272752\u003cbr\u003e\n- Anti-BMP9 antibody [EPR22898-266] - BSA and Azide free (Detector)\u003cbr\u003e\nab272752\u003cbr\u003e\nBone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2, is a protein encoded by the\u003cbr\u003e\ngene and expressed in the liver. BMP9 is a member of the TGF-? superfamily that plays a role in angiogenesis as a potent inhibitor as well as a potent regulator for osteogenesis, glucose metabolism, and vascular tone. Studies have shown that mutations in BMP9 cause vascular disorders. Mouse, rat, cow, monkey share 96.4%, 94.5%, 92.7%, and 97.3% sequence homology, respectively.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843575238825,"sku":"ab272470","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab272470","provider":"Iright","version":"1.0","type":"link"}