{"product_id":"abcam-ab272852","title":"Abcam, ab272852, Anti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric)","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nHuman Recombinant Monoclonal NCAP antibody. Suitable for ELISA, WB and reacts with SARS-CoV-2 samples. Cited in 8 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Human,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:1A6,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:SARS-CoV-2,\u003cbr\u003e\nApplications:WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-SARS-CoV-2 nucleocapsid protein antibody [1A6] - Human IgG1 (Chimeric) (ab272852) is a recombinant human\/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.\u003cbr\u003e\nAs the Fc region is human, an anti-Human secondary antibody should be used for detection.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Purification notes-Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe SARS-CoV-2 nucleocapsid protein also known as N protein plays a central role in the packaging and replication of the viral RNA genome. It functions mechanically by binding to the viral RNA to facilitate its replication and encapsidation. The nucleocapsid protein is expressed within the virus-infected cells particularly in the cytoplasm. It is a highly conserved structural protein of the virus and has a molecular weight of approximately 50 kDa. Additionally the nucleocapsid protein aids in RNA binding and is essential for the virus's structural stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe nucleocapsid protein of SARS-CoV-2 is involved in maintaining the integrity of the viral genome. It forms a ribonucleoprotein complex with the viral RNA which is necessary for viral replication. The nucleocapsid protein is integral in the formation of the virus's structure and assists in the assembly and release of new virus particles. It interacts with other viral proteins contributing to the stability and functioning of the virus.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe nucleocapsid protein is involved in the viral life cycle and replication pathways. The protein participates in the RNA replication and transcription complex working alongside proteins such as the RNA-dependent RNA polymerase and other non-structural proteins. These interactions within the pathways are essential for the efficient replication of the viral genome and production of new viral particles which are key steps in the virus's lifecycle.\u003cbr\u003e\nThe nucleocapsid protein is directly linked to COVID-19 the disease caused by the SARS-CoV-2 virus. It is a target for various diagnostic tests such as the COVID ELISA test and corona IgA test due to its immunogenic properties. The presence of nucleocapsid antibodies in patients is often measured through tests like the ELISA test which helps in diagnosing COVID-19. The nucleocapsid protein's involvement in COVID-19 progression connects it with other viral proteins highlighting its significance in disease mechanisms and potential therapeutic targeting.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844156936361,"sku":"ab272852","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab272852","provider":"Iright","version":"1.0","type":"link"}