{"product_id":"abcam-ab272854","title":"Abcam, ab272854, Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric)","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nHuman Recombinant Monoclonal SPIKE antibody. Suitable for ELISA, WB, FuncS (Neut\/Block) and reacts with SARS-CoV-2, SARS-CoV samples. Cited in 5 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Human,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:H6,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:SARS-CoV, SARS-CoV-2,\u003cbr\u003e\nApplications:FuncS (Neut\/Block), ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nSpecificity:We have in-house data that suggests this product also reacts with SARS-CoV-1 spike protein in Western blot.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) is a recombinant human\/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.\u003cbr\u003e\nAs the Fc region is human, an anti-Human secondary antibody should be used for detection. A suitable anti-Mouse secondary may also be used.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-undefined SDS-PAGE, Purification notes-Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe SARS-CoV-2 Spike Glycoprotein also known as COVID-19 Spike Protein is a trimeric protein weighing approximately 180-200 kDa. It is located on the surface of the SARS-CoV-2 virus playing an important role in virus infectivity. The protein comprises two main subunits S1 and S2 which facilitate attachment to and fusion with host cells. The S1 subunit contains the receptor binding domain (RBD) which specifically binds to the human angiotensin-converting enzyme 2 (ACE2) receptor. The Spike Glycoprotein is expressed in infected host cells mainly in the respiratory tract allowing the virus to enter and initiate replication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe SARS-CoV-2 Spike Glycoprotein initiates viral entry by interacting with the host cell's ACE2 receptor which leads to viral fusion and entry into the cell's cytoplasm. The protein is part of the virion structure and forms the visible spike on the virion's surface. Upon binding a conformational change triggers the fusion of viral and cellular membranes a vital step for the viral lifecycle. The multimeric nature of the Spike Protein facilitates its interaction with antibodies including those known as 'anti-spike antibodies' which can neutralize the virus and prevent cell infection.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe Spike Glycoprotein plays a significant role in the viral infection process and immune response pathways. It is central to the receptor-mediated endocytosis pathway where the virus is internalized into host cells. The protein’s interaction with ACE2 modifies downstream signaling pathways potentially altering host cell functions. Related proteins in these pathways include the ACE2 receptor and the cellular protease TMPRSS2 which primes the Spike Protein for fusion and viral entry.\u003cbr\u003e\nThe Spike Glycoprotein is chiefly implicated in COVID-19 the disease caused by SARS-CoV-2. The interaction with ACE2 is not just vital for infection but also contributes to the disease's symptomatology as ACE2 is involved in regulating blood pressure and inflammation. Additionally the Spike Protein's role in viral entry makes it a target for therapeutic interventions including 'anti-spike antibodies' and vaccines aimed at blocking this process to prevent infection. The protein's relevance to COVID-19 has led to significant interest in developing diagnostic and therapeutic tools such as 'antibodies COVID' that target the Spike Glycoprotein to manage the disease effectively.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844164112553,"sku":"ab272854","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab272854","provider":"Iright","version":"1.0","type":"link"}