{"product_id":"abcam-ab2732","title":"Abcam, ab2732, Anti-mTOR antibody","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nAnti-mTOR antibody (ab2732) is a rabbit polyclonal antibody detecting mTOR in  Western Blot, IP, ICC\/IF . Suitable for  Human, Rat . - Over 340 publications - Trusted since 2003\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human MTOR aa 200-250. The exact immunogen used to generate this antibody is proprietary information.P42345\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-mTOR antibody (ab2732) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of mTOR?\u003cbr\u003e\nAnti-mTOR (ab2732) specifically detects a band for mTOR (UniProt: P42345) at a molecular weight of 289kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-mTOR (ab2732) was first used in a scientific publication in 2003 and has been cited over 340 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-mTOR (ab2732) has over 10 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification notes-Affinity purified using the immunising peptideimmobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.1% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle, Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe mammalian target of rapamycin commonly known as mTOR is a serine\/threonine kinase known for its role in cellular growth and metabolism. It has a molecular weight of approximately 289 kDa. mTOR is expressed in various tissues throughout the body including muscle adipose tissue and the brain. The protein functions as a central regulator of cell proliferation protein synthesis and nutrient signaling. Often researchers utilize mTOR ELISA or mTOR western blot (mTOR WB) methods and mTOR antibodies to study its expression and activity in various biological contexts.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMTOR integrates signals from nutrients growth factors and cellular energy status to maintain cellular homeostasis. It forms part of two distinct complexes mTORC1 and mTORC2 which differ in their component proteins and downstream effects. mTORC1 primarily responds to amino acids and regulates protein synthesis through phosphorylation of key substrates like S6K1. On the other hand mTORC2 is important for maintaining cytoskeletal integrity and cell survival highlighting the protein's importance in diverse cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nMTOR plays a pivotal role in the PI3K\/AKT\/mTOR pathway which governs cell growth proliferation and survival. It also has implications in the regulation of the AMPK pathway which senses cellular energy levels. Through these pathways mTOR interacts with proteins such as AKT and TSC2. The phospho-mTOR specifically the S2448 phospho-mTOR serves as an important functional marker in these signaling cascades linking extracellular signals to downstream cellular responses.\u003cbr\u003e\nMTOR has connections to cancer and neurodegenerative diseases. Its dysregulation often leads to uncontrolled cellular proliferation a hallmark of many cancers. Conditions such as tuberous sclerosis can occur due to mutations in proteins like TSC1 and TSC2 that regulate mTOR activity. In Alzheimer's disease mTOR's role in autophagy and protein synthesis becomes significant as imbalance may contribute to disease progression. Understanding these connections highlights the potential of targeting mTOR pathways therapeutically.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855754678441,"sku":"ab2732","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab2732","provider":"Iright","version":"1.0","type":"link"}