{"product_id":"abcam-ab273306","title":"Abcam, ab273306, Aromatase (CYP19A) Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nAromatase Activity Assay Kit (Fluorometric) (ab273306) enables rapid measurement of native or recombinant aromatase activity in biological samples such as placental microsomes and S9 fractions. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Tissue Lysate, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAromatase Activity Assay Kit (Fluorometric) (ab273306) enables rapid measurement of native or recombinant aromatase activity in biological samples such as placental microsomes and S9 fractions.\u003cbr\u003e\nThe assay utilizes a fluorogenic substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex\/Em = 488\/527 nm), ensuring a high signal-to-background ratio with little interference by autofluorescence.\u003cbr\u003e\nA highly selective aromatase inhibitor is provided for determination of aromatase activity in heterogeneous biological samples, where other CYP isozymes may contribute to substrate metabolism. The inhibitor displays greater than 100-fold selectivity for aromatase over other enzymes, ensuring targeted inhibition. Aromatase specific activity is calculated by running parallel reactions in the presence and absence of the inhibitor and subtracting any residual activity detected with the inhibitor present.\u003cbr\u003e\nThe kit contains a complete set of reagents sufficient for 50 sets of paired reactions (in the presence and absence of inhibitor) and can detect a minimum of 25 nU aromatase activity.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K983 Aromatase (CYP19A) Activity Assay Kit (Fluorometric). K983-100 is the same size as the 100 test size of ab273306.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAromatase also known as CYP19A is an enzyme responsible for the conversion of androgens into estrogens. It is a member of the cytochrome P450 superfamily and has a molecular mass of approximately 58 kDa. Aromatase is mainly expressed in the gonads brain adipose tissue and placenta. The activity of this enzyme is important for estrogen biosynthesis which is especially important in postmenopausal women where adipose tissue becomes an important estrogen source.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme catalyzes the aromatization of androgens aiding in the production of estrogens like estradiol. This process is essential for maintaining estrogen levels in the human body. Aromatase operates as a monomer and does not form part of any larger complex. The balance of androgens and estrogens maintained by aromatase activity plays a significant role in reproductive functions and other physiological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nAromatase enzyme activity plays a pivotal role in the steroidogenesis pathway which involves the biosynthesis of steroid hormones. It is also a part of the estrogen signaling pathway interacting with other proteins like estrogen receptors. These pathways help regulate gene expression and are important in processes such as cell proliferation and differentiation linking aromatase function to broader metabolic and developmental effects.\u003cbr\u003e\nAromatase has a significant role in conditions such as hormone receptor-positive breast cancer and endometriosis. In breast cancer increased aromatase activity elevates estrogen levels promoting cancer cell growth. Anti-aromatase drugs like aromatase inhibitors target this enzyme to lower estrogen production. Additionally defects in aromatase activity can lead to disorders like aromatase deficiency resulting in an imbalance in sex hormone levels. Understanding its function aids in the development of therapies targeting proteins like the estrogen receptor which interacts with products of aromatase activity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855611449513,"sku":"ab273306","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab273306","provider":"Iright","version":"1.0","type":"link"}