{"product_id":"abcam-ab273506","title":"Abcam, ab273506, Anti-HAI-1 antibody [EPR23294-49] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal HAI-1 antibody. Carrier free. Suitable for IP, WB, ICC\/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR23294-49,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IP, Flow Cyt (Intra), IHC-P, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Unsuitable for human and rat IHC-P.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab273506 is the carrier-free version of\u003cbr\u003e\nab272721\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHAI-1 known also as Hepatocyte Growth Factor Activator Inhibitor 1 or SPINT1 is a type 1 transmembrane protein with a molecular mass of around 55 kDa. This protein commonly expresses in epithelial cells particularly in tissues like the stomach and liver. It serves as a serine protease inhibitor playing a role in regulating the activity of certain proteases. Mouse HAI protein comparable in function and structure is often used in research as a model for understanding HAI-1 in humans. Researchers frequently use HAI detectors and HAI-1 ELISA methods for precise quantification and analysis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHAI-1 performs a regulatory role by inhibiting proteases such as matriptase and hepsin which are critical for many cellular functions. As part of a complex HAI-1 binds to these proteases controlling their activity to prevent excessive proteolysis which can damage tissues. This regulatory function is key in maintaining epithelial integrity and homeostasis impacting processes like cell growth and migration. Anti-HAI studies show that disrupting this balance can lead to a cascade of biological alterations.\u003cbr\u003e\nPathways\u003cbr\u003e\nSeveral cellular processes heavily depend on the balance maintained by HAI-1 in protease activity. One significant pathway involves the HGF\/c-Met signaling cascade which impacts cell motility and proliferation. By inhibiting matriptase HAI-1 indirectly affects this pathway regulating cellular responses. Additionally HAI-1 also relates to epithelial-mesenchymal transition (EMT) through controlling proteolytic activity which can influence how cells migrate and differentiate in development or healing.\u003cbr\u003e\nDeregulation of HAI-1 often links to cancer progression specifically in cases like breast or prostate cancer. In these contexts improper regulation of proteases can lead to enhanced tumor invasiveness and metastasis. HAI-1's interaction with the c-Met receptor pathway facilitated by HGFA illustrates its significant role in cancer biology. Disorders of the liver such as fibrosis also reveal connections where HAI-1 modulation of protease activity affects extracellular matrix turnover implicating HAI-1 in the fibrogenic response alongside proteins like matriptase.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850770501801,"sku":"ab273506","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab273506","provider":"Iright","version":"1.0","type":"link"}