{"product_id":"abcam-ab273854","title":"Abcam, ab273854, Human IFI16 knockout Jurkat cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial \/ 2 x 1000000Cells \/ vial\u003cbr\u003e\nIFI16 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR\/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:Jurkat,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Blood,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by CRISPR\/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%,\u003cbr\u003e\nDisease:Non-Hodgkin Lymphoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-IFI16, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions--196°C, -80°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIfi204 also known as Interferon-activated gene 204 is a protein of the mouse involved in the immune response. It has a molecular mass of approximately 74 kilodaltons. This protein is expressed mainly in activated macrophages and neutrophils and contributes to the innate immune mechanism. It detects cytosolic DNA and activates the STING pathway to induce type I interferon production. It contains a HIN-200 domain that helps in recognizing specific DNA sequences.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nInterferon-activated gene 204 plays a role in the host defense mechanism. It functions as part of the cellular response to viral infections and potentially some bacterial infections. The protein is part of a larger complex involved in DNA sensing and it activates immune signaling pathways upon detecting foreign DNA. It interacts with components of the inflammasome participating in activating inflammatory responses necessary for pathogen clearance.\u003cbr\u003e\nPathways\u003cbr\u003e\nInterferon-activated gene 204 participates in the cGAS-STING DNA sensing pathway. This pathway is essential for the production of type I interferons as a defense against viruses. The protein associates with cGAS and STING key players in the recognition of cytosolic DNA. Its role in this pathway highlights its importance in the body's first line of defense closely linking it to the activation of downstream signaling that involves IRF3 and NF-kB.\u003cbr\u003e\nAltered expression or function of Interferon-activated gene 204 has connections with autoimmunity and inflammatory diseases. Its dysregulation may lead to excessive inflammation contributing to conditions like systemic lupus erythematosus. In this context it interacts with proteins such as AIM2 which is part of the inflammasome complex involved in autoimmune inflammation. Understanding its precise role in these diseases could potentially lead to new therapeutic strategies for managing autoimmune disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845148266665,"sku":"ab273854","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab273854","provider":"Iright","version":"1.0","type":"link"}