{"product_id":"abcam-ab2739","title":"Abcam, ab2739, Anti-O-Linked N-Acetylglucosamine antibody [RL2]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) is a mouse monoclonal antibody detecting O-Linked N-Acetylglucosamine in  Western Blot, ICC\/IF . Suitable for  Human, Rat . - Over 180 publications - Trusted since 2003\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:RL2,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Rat, Human,\u003cbr\u003e\nApplications:ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThis antibody clone [RL2] is manufactured by Abcam.\u003cbr\u003e\nIf you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact\u003cbr\u003e\norders@abcam.com\u003cbr\u003e\nor you can find further information\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) is a mouse monoclonal antibody and is validated for use in ICC\/IF, WB in human, rat samples.\u003cbr\u003e\nAnti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) specifically detects O-Linked N-Acetylglucosamine (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) has high sensitivity and specificity.\u003cbr\u003e\nAnti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) has been cited over 186 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) has 17 independent reviews from customers.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone RL2 -\u003cbr\u003e\nab288303\u003cbr\u003e\nAntibody clone RL2 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP (\u003cbr\u003e\nab201993\u003cbr\u003e\nab201994\u003cbr\u003e\nab201995\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nO-Linked N-Acetylglucosamine (O-GlcNAc) is a post-translational modification involving the addition of a single N-acetylglucosamine moiety to the serine or threonine residues of nuclear and cytoplasmic proteins. This dynamic modification is sometimes referred to as O-GlcNAcylation. The molecular mass of the O-GlcNAc group itself is approximately 203 Da. This modification is expressed widely across various tissues notably in the brain and pancreas. O-GlcNAc plays a critical role in regulating protein function stability and interaction.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nO-GlcNAc modifies proteins influencing cellular processes such as transcription signal transduction and stress response. It is involved in the regulation of transcription factors like Sp1 and NF-kB and is associated with the O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) enzymes which respectively add and remove the GlcNAc group. O-GlcNAc functions in a manner similar to phosphorylation often competing with it on the same or adjacent serine\/threonine sites. This modification is not part of a permanent protein complex but dynamically modulates protein interactions and activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nThis modification plays a fundamental role in pathways related to nutrient sensing and insulin signaling. It modulates proteins such as insulin receptor substrate 1 (IRS1) and Akt to link nutrient status to cellular responses. The hexosamine biosynthetic pathway (HBP) is an important pathway in which O-GlcNAc is synthesized. Through these pathways it impacts cellular signaling and metabolism influencing processes like cellular growth and apoptosis.\u003cbr\u003e\nO-GlcNAc modification connects to conditions like diabetes and Alzheimer's disease. Elevated O-GlcNAc levels contribute to insulin resistance a hallmark of type 2 diabetes through interaction with proteins such as IRS1 and Akt. In Alzheimer's O-GlcNAc modifies tau protein reducing its phosphorylation and aggregation therefore potentially affecting neurofibrillary tangle formation. These connections underline the importance of O-GlcNAc in the pathophysiology of these diseases and highlight its potential as a therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844219883689,"sku":"ab2739","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab2739","provider":"Iright","version":"1.0","type":"link"}