{"product_id":"abcam-ab275552","title":"Abcam, ab275552, Human PGC ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman PGC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PGC with a sensitivity of 16.5 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 16.5 pg\/mL,\u003cbr\u003e\nRange:54.69 - 3500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman PGC ELISA kit (ab275552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PGC protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PGC with 16.5 pg\/mL sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nPepsinogen C (PGC, progastricsin) is an aspartic proteinase zymogen that belongs to the peptidase family A1. Pespsin C, the activated protease, is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. Pepsinogen C protein is synthesized as an inactive zymogen that autocatalizes by sequential cleavage of the prosegment into its active mature form at low pH. Pepsinogen C polymorphisms are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPepsinogen II also known as PGC or progastricsin functions as a digestive zymogen activated to pepsin in the acidic environment of the stomach. This enzyme precursor has a molecular mass of approximately 42-47 kDa. It is primarily expressed in the gastric chief cells and mucous cells of the stomach lining. Pepsinogen II plays an important role in the initial digestion of proteins breaking down complex protein molecules into peptides.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPepsinogen II contributes to digestive processes by converting into active pepsin at low pH levels facilitating protein degradation. Unlike some other digestive enzymes pepsin works independently from a complex. The production and secretion of pepsinogen II align with the gastric acid production ensuring efficient digestive activity. Its expression is vital for proper nutritional absorption and maintenance of gastrointestinal health.\u003cbr\u003e\nPathways\u003cbr\u003e\nPepsinogen II is closely involved in the proteolytic enzyme pathway. It connects with other gastric enzymes such as pepsin A through the activation processes influenced by gastric acid secretion. This interplay among digestive enzymes ensures optimal protein breakdown and nutrient absorption. The regulatory mechanisms that modulate gastric acid and pepsinogen secretion play an integral role in maintaining the digestive efficiency.\u003cbr\u003e\nPepsinogen II levels can serve as biomarkers for certain gastric conditions including gastritis and gastric cancer. Altered levels of pepsinogen II are associated with atrophic gastritis where reduced enzyme levels reflect damage to gastric mucosa. Also increased serum pepsinogen II concentration can indicate potential gastric cancer risk. These conditions highlight pepsinogen II's clinical importance in gastrointestinal disease diagnosis and management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843563507881,"sku":"ab275552","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab275552","provider":"Iright","version":"1.0","type":"link"}