{"product_id":"abcam-ab275755","title":"Abcam, ab275755, pCambia2301 Plant Expression Vector","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nVector contains genes for bacterial and plant selection with kanamycin and the gusA reporter gene.\u003cbr\u003e\nKey facts\u003cbr\u003e\nTarget:pCambia2301See target data,\u003cbr\u003e\nForm:LyophilizedSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: 71% Tris, 17% EDTA\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nVector contains genes for bacterial and plant selection with kanamycin and the\u003cbr\u003e\nreporter gene.\u003cbr\u003e\nThe pCambia2301 vector offers:\u003cbr\u003e\nHigh copy number in\u003cbr\u003e\nE.coli\u003cbr\u003e\nfor high DNA yields\u003cbr\u003e\npVS1 replicon for high stability in\u003cbr\u003e\nAgrobacterium\u003cbr\u003e\nSmall size\u003cbr\u003e\nRestriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest\u003cbr\u003e\nBacterial selection with kanamycin\u003cbr\u003e\nPlant selection with kanamycin\u003cbr\u003e\nSimple means to construct translational fusions to\u003cbr\u003e\nreporter genes\u003cbr\u003e\nPlant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV\u003cbr\u003e\nS promoter and terminated by the CaMV\u003cbr\u003e\nS polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.\u003cbr\u003e\nThis vector contains a fully functional\u003cbr\u003e\nreporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses\u003cbr\u003e\nE.coli gusA\u003cbr\u003e\nwith an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual\u003cbr\u003e\nA.tumefaciens\u003cbr\u003e\ncells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the\u003cbr\u003e\ngene and insert their own gene of interest in its place or use this vector to create fusions of\u003cbr\u003e\nwith their gene of interest. This vector contains the pUC18 polylinker-\u003cbr\u003e\nGenbank Sequence data\u003cbr\u003e\nConcentration of Antibiotics for selection.\u003cbr\u003e\nIn bacterial system:\u003cbr\u003e\nKanamycin resistance (\u003cbr\u003e\naphIII\u003cbr\u003e\nnptIII\u003cbr\u003e\n\/3'5''-aminoglycoside phosphotransferase typeIII from\u003cbr\u003e\nEnterococcus faecalis\u003cbr\u003e\npJH1) in bacteria: use 50 μg\/mL in\u003cbr\u003e\nE.coli\u003cbr\u003e\nA.tumefaciens\u003cbr\u003e\nIn plant system:\u003cbr\u003e\nResistance Gene\u003cbr\u003e\nnptII\/aph(3')II\u003cbr\u003e\nfrom Tn5\u003cbr\u003e\nTransformed plant selection antibiotic\u003cbr\u003e\nKanamycin\u003cbr\u003e\nConcentration in\u003cbr\u003e\nArabidopsis\u003cbr\u003e\n20 - 50 μg\/mL\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847580602537,"sku":"ab275755","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab275755","provider":"Iright","version":"1.0","type":"link"}