{"product_id":"abcam-ab276141","title":"Abcam, ab276141, Anti-MICA + MICB antibody [EPR24086-121] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal MICA antibody. Carrier free. Suitable for IP, Flow Cyt, WB and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR24086-121,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IP, Flow Cyt, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab276141 is the carrier-free version of\u003cbr\u003e\nab259934\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMICA and MICB also referred to as Mhc class I polypeptide-related sequence A and B are stress-induced proteins and are known to have a molecular weight of approximately 62 kDa and 70 kDa respectively. They are expressed on the surface of many cell types especially epithelial cells fibroblasts and upon certain cancers. These proteins are encoded within the MHC class I region yet unlike classic MHC class I molecules they do not associate with beta-2-microglobulin and they do not present antigens to T cells. Instead MICA and MICB can activate immune responses by engaging the NKG2D receptor on natural killer (NK) cells CD8+ T cells and gamma-delta T cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nMICA and MICB contribute to the immune surveillance system by marking stressed or transformed cells for destruction. These proteins are not part of a complex but are known ligands for the NKG2D receptor. Their interaction with NKG2D triggers the cytotoxic activity of NK cells and CD8+ T cells enhancing the destruction of cancerous or infected cells. Especially interesting is their role in tumor immunology where they serve as a danger signal to the immune system pointing NK cells toward tumor cells that would otherwise evade detection.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role of MICA and MICB centers around the immune response and cell stress pathways. One important pathway is the stress-induced signal transduction which upregulates these proteins in response to cellular stress such as heat shock. They fit into the immune surveillance pathway interacting with proteins like the NKG2D receptor and other signaling molecules that modulate immune cell activation. The pathway emphasizes the removal of damaged or abnormal cells and this interaction orchestrates timely immune responses.\u003cbr\u003e\nMICA and MICB have associations with certain cancers like liver and colorectal cancers where they become over-expressed and shed from tumor cells to escape immune detection. Additionally their presence links to autoimmune diseases such as rheumatoid arthritis where the immune system attacks the body's own cells unjustly. The shedding of MICA\/MICB from tumor cells involves enzymes like metalloproteinases affecting the engagement of NKG2D and complicating the immune response to cancerous cells.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851267723433,"sku":"ab276141","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab276141","provider":"Iright","version":"1.0","type":"link"}