{"product_id":"abcam-ab27671","title":"Abcam, ab27671, Anti-V5 tag antibody [SV5-Pk1]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-V5 tag antibody [SV5-Pk1] (ab27671) is a mouse monoclonal antibody detecting V5 tag in  Western Blot, Flow Cytometry, IP, IHC-Fr, ELISA . - Over 150 publications - Trusted since 2005\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:SV5-Pk1,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:RIA, WB, IHC-Fr, ELISA, Flow Cyt, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nEpitope:ab27671 recognises a small epitope, termed Pk, present on the P\/V proteins of the paramyxovirus, SV5.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-V5 tag antibody [SV5-Pk1] (ab27671) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt, IHC-Fr, IP, RIA, WB in human, mouse samples.\u003cbr\u003e\nAnti-V5 tag antibody [SV5-Pk1] (ab27671) has been cited over 153 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-V5 tag antibody [SV5-Pk1] (ab27671) has high sensitivity and specificity.\u003cbr\u003e\nAnti-V5 tag antibody [SV5-Pk1] (ab27671) has 11 independent reviews from customers.\u003cbr\u003e\nAnti-V5 tag antibody [SV5-Pk1] (ab27671) specifically detects V5 tag (UniProt ID: P11207; Molecular weight: 24kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nAntibody clone SV5-Pk1 is also available pre-conjugated to Biotin for your convenience (\u003cbr\u003e\nab27672\u003cbr\u003e\nab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Purification notes-This antibody was purified from tissue culture supernatant by affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nResearchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.\u003cbr\u003e\nScientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844230893737,"sku":"ab27671","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab27671","provider":"Iright","version":"1.0","type":"link"}