{"product_id":"abcam-ab277407","title":"Abcam, ab277407, Mouse galectin 9\/Gal-9 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse galectin 9\/Gal-9 ELISA Kit is a Quantitative ELISA for the measurement of Mouse galectin 9\/Gal-9 in Mouse in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:= 0.18 ng\/mL,\u003cbr\u003e\nRange:0.164 - 40 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse galectin 9\/Gal-9 ELISA kit (ab277407) is an\u003cbr\u003e\nin-vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Mouse galectin 9\/Gal-9 in serum, plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Mouse galectin 9\/Gal-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse galectin 9\/Gal-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse galectin 9\/Gal-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse galectin 9\/Gal-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGalectin-9 also known as Gal-9 or LGALS9 is a β-galactoside-binding lectin with a molecular mass of around 40 kDa. It shows expression in various tissues including the thymus spleen and liver. The galectin-9 protein is involved in cell-cell and cell-matrix interactions where it binds to glycoproteins and glycolipids. Researchers commonly use methods like Galectin-9 ELISA to measure its levels in different biological samples.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGalectins like galectin-9 play essential roles in regulating diverse immune responses. Galectin-9 acts within the immune system by modulating inflammation and apoptosis. It acts as both a pro-apoptotic factor for T cells and a protein mediator in innate immune functions. Galectin-9 proteins can form complexes with other lectins and cell surface receptors contributing to cellular signaling processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nGalectin-9 is integral to the immune system's functional networks. It participates in the T-cell regulation pathways and the innate immunity pathways. In these pathways it interacts with other proteins like TIM-3 which mediates T-cell apoptosis and contributes to immune tolerance. These interaction networks play important roles in maintaining the body's immune homeostasis.\u003cbr\u003e\nGalectin-9 has associations with autoimmune diseases and cancer. Its regulatory effects on T cells connect it to autoimmune conditions where abnormal T-cell activation occurs. In cancer the alteration in galectin-9 expression levels affects tumor immunity. The interaction of galectin-9 with other proteins like TIM-3 is important in cancer progression making it a target of interest for therapeutic interventions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855637729449,"sku":"ab277407","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab277407","provider":"Iright","version":"1.0","type":"link"}